Introduction:Folliclestimulatinghormone(FSH)isahormonesynthesisedandsecretedbygonadotropesintheanteriorpituitarygland.FSHandLHactsynergisticallyinreproduction:Inwomen,intheovaryFSHstimulatesthegrowthofimmatureGraafianfolliclestomaturation.Asthefolliclegrowsitreleasesinhibin,whichshutsofftheFSHproduction.Inmen,FSHenhancestheproductionofandrogen-bindingproteinbytheSertolicellsofthetestesandiscriticalforspermatogenesis.Inbothmalesandfemales,FSHstimulatesthematurationofgermcells.Infemales,FSHinitiatesfolliculargrowth,specificallyaffectinggranulosacells.WiththeconcomitantriseininhibinBFSHlevelsthendeclineinthelatefollicularphase.Thisseemstobecriticalinselectingonlythemostadvancedfollicletoproceedtoovulation.Attheendofthelutealphase,thereisaslightriseinFSHthatseemstobeofimportancetostartthenextovulatorycycle.Likeitspartner,LH,FSHreleaseatthepituitaryglandiscontrolledbypulsesofgonadotropin-releasinghormone(GnRH).Thosepulses,inturn,aresubjecttotheestrogenfeed-backfromthegonads.Description:FSHHumanRecombinantproducedinHEK-293cellsisheterodimeric,glycosylated,polypeptidechaintransfectedwithtwoexpressionplasmidsencodingthehumanFSH-alphachain(Accession#P01215)(Ala25-Ser116)andhumanFSH-betachain(Asn19-Glu129)(Accession#P01225)havingatotalMwof38kDa.FSHhumanrecombinantispurifiedbyproprietarychromatographictechniques.PhysicalAppearance:SterileFilteredWhitelyophilized(freeze-dried)powder.Formulation:TherecombinantFSHwaslyophilizedfrom0.2µmfilteredsolutioncontainingPBS,pH7.4.Solubility:ItisrecommendedtoreconstitutethelyophilizedFollicleStimulatingHormoneinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.StABIlity:LyophilizedrecombinantFSHalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFSHshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.Purity:Greaterthan95%asdeterminedbySDS-PAGE.BIOLOGicalActivity:TheED50asdeterminedbycAMPaccumulationinhumanFSHReceptortransfectedChineseHamsterOvarycellswasfoundtobe80-450pg/ml.ForLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.